14562
Sigma
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester
BioChemika, for fluorescence
| Synonym: | BCECF-AM |
| Linear Formula: | C39H36O19 |
| Molecular Weight: | 808.69 |
| MDL number: | MFCD01862636 |
Description
| Application | Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells. |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| fluorescence | λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase) |
| solubility | DMF: soluble |
| DMSO: soluble | |
| acetonitrile: soluble | |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | Muallem, S., Simultaneous recording of cell volume changes and intracellular pH or Ca2+ concentration in single osteosarcoma cells UMR-106-1. J. Biol. Chem. 267, 17658, (1992) |
| K. Inouye, B. van Duijn, A. Wiltink, eds. Signal Transduction - Single Cell Techniques, (1998), 375 |
